Fig. 3: DEPTOR displacement from mTORC1 increases the association between raptor and IRS-1.
From: Differential regulation of mTORC1 and mTORC2 is critical for 8-Br-cAMP-induced decidualization
a, b Human endometrial stromal (hES) cells were induced to differentiate for 4 days in the presence of 0.5 mM 8-Br-cAMP. On day 0, 2, or 4 of differentiation, the cells were lysed and subjected to western blotting (a) and quantitative RT-PCR (b) to analyze DEPTOR expression. Human GAPDH was used to normalize gene expression. c On day 0 or 2 of differentiation, hES cells were lysed, subjected to immunoprecipitation against mTOR, and analyzed by western blotting. d, e Cells were treated as in (c), lysed, subjected to immunoprecipitation against either raptor (d) or rictor (e), and then analyzed by western blotting. f Cells were treated with 300 μM C8-PA for 30 min, lysed, subjected to immunoprecipitation against raptor, and analyzed by western blotting. All data are shown as the mean ± SD or are blots representative of three to five independent experiments. Student’s t-tests were performed to compare the indicated pairs of data. *P < 0.05
