Fig. 5: Differential regulation of mTORC1 and mTORC2 inactivates Akt, accompanied by decreased pS256-FOXO1 during 8-Br-cAMP-induced decidualization.

a Human endometrial stromal (hES) cells were differentiated with 8-Br-cAMP for 4 days, lysed, and analyzed by western blotting. b On day 0 or 2 of 8-Br-cAMP-induced differentiation, hES cells were lysed, subjected to immunoprecipitation against rictor, and analyzed by western blotting. c–e Cells were infected with rictor shRNAs (c), raptor shRNAs (d), DEPTOR shRNAs (e), or scrambled shRNA; cells were then selected by puromycin for 5 days. Cells were differentiated in the presence of 0.5 mM 8-Br-cAMP, lysed, and then subjected to western blotting. f Cells were transfected with Flag-DEPTOR, differentiated with 0.5 mM 8-Br-cAMP for 2 days, lysed, and then subjected to western blotting. All blots are representative of three to five independent experiments