Fig. 6: NaB-enhanced GLP-1R expression in HepG2 cells via HDAC inhibition that was independent of GPR43 or GPR109a.

a Western blot analysis of GLP-1R protein expression in siRNA-transfected HepG2 cells that were cotreated with FFA (0.5 mM) and NaB (5 mM) for 24 h. b–e Western blot analysis of total acetyl-histone levels in HepG2 cells that were treated with increasing concentrations of FFA for 24 h (b), or in HepG2 cells that were treated with FFA (0.5 mM) for 0 to 48 h (c), or in HepG2 cells that were treated with NaB alone (1–10 mM) for 24 h (d), or in HepG2 cells that were cotreated with FFA (0.5 mM) and NaB for 24 h (e). f Western blot analysis of total acetyl-histone levels in siRNA-transfected HepG2 cells that were cotreated with FFA (0.5 mM) and NaB. The data represent the means ± S.E.M.; vs. control **P < 0.01 and ***P < 0.001; vs. FFA ^##P < 0.01 and ^###P < 0.001