Fig. 4: ETV2 overexpression increases capillary density in MI hearts. | Experimental & Molecular Medicine

Fig. 4: ETV2 overexpression increases capillary density in MI hearts.

From: In vivo transduction of ETV2 improves cardiac function and induces vascular regeneration following myocardial infarction

Fig. 4

MI hearts of mice receiving lentiviral particles of ETV2-Ires-VENUS or VENUS were perfused with rhodamine conjugated IB4 to visualize vessels. They were subjected to immunohistochemical staining with anti-GFP antibody, IB4 (red), ETV2 (green), and DAPI for nuclei (blue). a Representative images of capillaries 8 weeks after MI (upper panels) and summarized quantification (lower panels), scale bars: 100 µm. For quantification, the number of capillaries in five randomly selected fields in each heart was counted, n = 5–7. b Representative images of capillaries (upper panels) and summarized quantification (lower panels) in the border zone and infarct zone in individual test groups, scale bars: 100 µm. n = 5. c A significant number of vessels were double-positive for both IB4 (red) and ETV2 (green) in ETV2-injected heart tissues. Representative confocal images of double-positive capillaries (upper panels) and summarized quantification (lower panels) in heart tissue harvested 8 weeks after ETV2 injection, scale bars: 50 µm. *p < 0.05, **p < 0.01 compared to control group

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