Fig. 3: Collagen production was induced in LOX- or LOXL2-downregulated preeclamptic placenta and trophoblast cells. | Experimental & Molecular Medicine

Fig. 3: Collagen production was induced in LOX- or LOXL2-downregulated preeclamptic placenta and trophoblast cells.

From: Downregulation of lysyl oxidase and lysyl oxidase-like protein 2 suppressed the migration and invasion of trophoblasts by activating the TGF-β/collagen pathway in preeclampsia

Fig. 3

a Representative hematoxylin-eosin staining and Masson’s trichrome staining of placenta and villi are shown. b Amount of total collagen was determined by measuring the hydroxyproline contents of placental tissues. c Western blot analysis of type I collagen, type III collagen, and type IV collagen in the placenta. d Statistical analysis of protein densitometry quantification of western blot analysis (c) by Student’s t-test. Data are presented as the means ± SEM. e Amount of soluble collagen from cell culture supernatants was quantified using a sircol collagen assay. Data are presented as the means ± SEM of three independent experiments. f Quantification of COL1A1, COL3A1, and COL4A1 mRNA expression levels determined in HTR-8/SVneo cells after siRNA treatment. Data are presented as the means ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ***P < 0.001; and NS not significant

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