Fig. 5: Inhibiting Smad3 could partially rescue trophoblast cell migration and invasion. | Experimental & Molecular Medicine

Fig. 5: Inhibiting Smad3 could partially rescue trophoblast cell migration and invasion.

From: Downregulation of lysyl oxidase and lysyl oxidase-like protein 2 suppressed the migration and invasion of trophoblasts by activating the TGF-β/collagen pathway in preeclampsia

Fig. 5

a Western blot analysis of pSmad3 and Smad3 in HTR-8/SVneo cells treated with the Smad3 inhibitor SIS3 (0, 2, 5, or 10 μmol/L) for 24 h. b Amount of soluble collagen in culture supernatants from HTR-8/SVneo cells transfected with small interfering RNA (siRNA) followed by SIS3 treatment (10 μmol/L) was quantified using a sircol collagen assay. c Quantification of COL1A1, COL3A1, and COL4A1 mRNA expression levels was determined in HTR-8/SVneo cells upon siRNA transfection followed by SIS3 treatment (10 μmol/L). d Migration and invasion of HTR-8/SVneo cells upon siRNA transfection followed by SIS3 treatment (10 μmol/L) was determined by Transwell assays. Representative images are shown. e Relative fold changes in cell migration and invasion were calculated. Statistical data were analyzed by Student’s t-test. Data are presented as the means ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001

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