Fig. 1: Different effects of TNF-α-induced miR-31 and miR-155 expression on NO and cGMP production in HUVECs. | Experimental & Molecular Medicine

Fig. 1: Different effects of TNF-α-induced miR-31 and miR-155 expression on NO and cGMP production in HUVECs.

From: NF-κB-responsive miR-155 induces functional impairment of vascular smooth muscle cells by downregulating soluble guanylyl cyclase

Fig. 1

HUVECs were transfected with 80 nM of control miRNA (C), miR-31 mimic (31), or miR-155 mimic (155) and then stimulated with or without TNF-α (10 ng/mL) for 24 h. a The NO2- levels were determined in culture supernatants by the Griess reaction (n = 4). b The cellular cGMP levels were determined using a cGMP assay kit (n = 4). c, d HUVECs were transfected with 80 nM of a specific control (C) for miR-155 mimic or miR-155 inhibitor, miR-155 mimic (M), or miR-155 inhibitor (I) and stimulated with or without TNF-α (10 ng/mL) for 24 h. The protein and mRNA levels of sGCα1 and sGCβ1 were measured by Western blotting and qRT-PCR (n = 4). *P < 0.05 and **P < 0.01

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