Fig. 5: TNF-α-induced miR-155 exerts VSMC phenotypic switching. | Experimental & Molecular Medicine

Fig. 5: TNF-α-induced miR-155 exerts VSMC phenotypic switching.

From: NF-κB-responsive miR-155 induces functional impairment of vascular smooth muscle cells by downregulating soluble guanylyl cyclase

Fig. 5

HASMCs were transfected with 80 nM of control miRNA and siRNA (C), miR-155 mimic (M), miR-155 inhibitor (I), or sGCβ1 siRNA (S) and stimulated with or without TNF-α (10 ng/mL) for 24 h, followed by treatment with DETA/NO (100 μM) for another 24 h. a–e The mRNA and protein levels of VSMC-specific marker genes were determined by qRT-PCR (n = 6) and Western blotting. f Cell proliferation was determined by a [3H]-thymidine incorporation assay (n = 3). g The migration distance was calculated as the average distance between the edges of the scratch field (n = 5). *P < 0.05, and **P < 0.01

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