Fig. 1: Altered gut microbiome and functional metabolic pathways in UUO rats. | Experimental & Molecular Medicine

Fig. 1: Altered gut microbiome and functional metabolic pathways in UUO rats.

From: Unilateral ureteral obstruction causes gut microbial dysbiosis and metabolome disorders contributing to tubulointerstitial fibrosis

Fig. 1

a, b Shannon_2 and Simpson of α-diversity index of colonic luminal content 16S rDNA sequencing data from UUO and sham rats after two weeks. The boxplots indicate the smallest and largest values, 25 and 75% quartiles, medians, and outliers. c, d Principal coordinates analysis of unweighted and weighted UniFrac distance based on 16S rDNA profiling (OTUs level) of colonic luminal content from sham and UUO rats. P values indicated differential clustering when assessed by ADONIS test. e Cladogram showing different abundant taxa between samples from sham and UUO rats using phylum to genus-level data. f, g Taxonomic distributions of bacteria from colonic luminal content 16S rDNA sequencing data at the phylum (top 10) and genus (top 30) levels between the UUO and sham groups. Wilcoxon rank-sum test was used to determine significance in α-diversity. h Modifications of the gut microbiota affect predicted functional metabolic pathways. Heatmap of metabolic pathways of the UUO and sham groups obtained from PICRUSt analysis of 16S rRNA sequencing data. Yellow and blue text highlights indicate upregulation and downregulation in the UUO rats versus the sham rats. Asterisks denote statistical significance between the sham and UUO groups (n = 6), *P < 0.05, **P < 0.01

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