Fig. 7: Effects of ergone and PU on inflammation and TIF in UUO rats. | Experimental & Molecular Medicine

Fig. 7: Effects of ergone and PU on inflammation and TIF in UUO rats.

From: Unilateral ureteral obstruction causes gut microbial dysbiosis and metabolome disorders contributing to tubulointerstitial fibrosis

Fig. 7

a Western blot depicting IκBα, p-IκBα, the nuclear content of p65, the active subunit of NF-κB and expression of COX-2 and MCP-1 in kidney tissues of the different rats. Tissue lysates were immunoblotted with specific antibodies against IκBα, p-IκBα, NF-κB, COX-2, and MCP-1. b Graphic representations of IκBα, p-IκBα, NF-κB, COX-2, and MCP-1 expression in different groups, as indicated. c Western blot depicting nuclear translocation of Nrf2 and protein abundances of its repressor, Keap1, and expression of its downstream gene products, catalase, HO-1 and NQO-1, in kidney tissues of the different rats. Tissue lysates were immunoblotted with specific antibodies against Keap1, Nrf2, catalase, HO-1, and NQO-1. d Graphic representations of Keap1, Nrf2, catalase, HO-1, and NQO-1 expression in the different groups, as indicated. e Images of PAS staining, Masson’s Trichrome staining and immunohistochemical staining of α-SMA, collagen I, and fibronectin expression in the different rats. f Graphic representations of PAS staining, Masson’s Trichrome staining and immunohistochemical staining in the different groups, as indicated. ERG ergone, PU, Polyporus umbellatus. *P < 0.05; **P < 0.01 versus sham rats (n = 6); #P < 0.05, ##P < 0.01 versus UUO rats (n = 6)

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