Fig. 4: The involvement of TBK1 in radiation-induced EMT occurs through the AKT signaling pathway. | Experimental & Molecular Medicine

Fig. 4: The involvement of TBK1 in radiation-induced EMT occurs through the AKT signaling pathway.

From: Blocking TBK1 alleviated radiation-induced pulmonary fibrosis and epithelial-mesenchymal transition through Akt-Erk inactivation

Fig. 4

a, b The expression levels of AKT-pS473 and AKT-pT308 in TBK1 knockdown RLE-6TN cells treated with or without 8 Gy irradiation were detected via western blot analysis. GAPDH and AKT were used as loading controls. c RLE-6TN cells were incubated with/without PF-04691502 for 2 h before 8 Gy irradiation. Cell lysates were collected, and the protein levels of AKT-pS473, AKT-pT308, and TBK1 at 1 h postirradiation were measured via western blot. GAPDH and AKT were used as loading controls. The data are presented as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. control group

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