Fig. 5: The ERK signaling pathway accounts for the regulatory effects of TBK1 in radiation-induced EMT | Experimental & Molecular Medicine

Fig. 5: The ERK signaling pathway accounts for the regulatory effects of TBK1 in radiation-induced EMT

From: Blocking TBK1 alleviated radiation-induced pulmonary fibrosis and epithelial-mesenchymal transition through Akt-Erk inactivation

Fig. 5

a, b Representative western blots and densitometric quantification of phosphorylated ERK at different times and with different radiation doses. c–e RLE-6TN cells were incubated with/without the selective ERK inhibitor SCH772984 for 2 h before irradiation with 8 Gy. c, e Western blot analysis of p-ERK, E-cadherin, vimentin, and α-SMA at 1 or 48 h postirradiation. d Phase contrast microscopy images of cells at 48 h after treatment with 8 Gy irradiation. Scale bar represents 100 μm. f The expression level of p-ERK in TBK1 knockdown RLE-6TN cells treated with or without 8 Gy irradiation was detected by western blot analysis. g RLE-6TN cells were incubated with/without SCH772984 for 2 h before irradiation with 8 Gy. Cell lysates were collected, and the protein levels of p-ERK and TBK1 at 3 h postirradiation were measured by western blot. GAPDH and ERK were used as loading controls. The data are presented as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. nonirradiated control

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