Fig. 2: A double proteomic approach reveals a role for the CDK16/CCNY complex in cytoskeletal regulation. | Experimental & Molecular Medicine

Fig. 2: A double proteomic approach reveals a role for the CDK16/CCNY complex in cytoskeletal regulation.

From: Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex

Fig. 2

a Strategy used to identify CCNY interactors using MCF7 cell extracts. The graphical representation of GO terms obtained by STRING analysis for biological processes and cellular components is shown below. Data were filtered with a p-value cutoff of 0.05 and a fold change cutoff of log2(3.23) and seen in all three biological replicates. b Chemical genetic approach based on the labeling of AS-GST-CDK16 substrates in HeLa cell extracts. The corresponding graphical representation of GO terms obtained by STRING analysis is shown below. Peptides that were present in the AS-CDK16 proteomic analysis but in neither the WT-CDK16 nor the negative control analyses were selected. The significance threshold for the false discovery rate (FDR) was set at ≥0.01. MS, mass spectrometry; N6-Ph-ATPγS, bulky ATP analog

Back to article page