Fig. 3: Identification of PRC1 as a direct substrate of the CDK16/CCNY complex. | Experimental & Molecular Medicine

Fig. 3: Identification of PRC1 as a direct substrate of the CDK16/CCNY complex.

From: Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex

Fig. 3

a The CDK16 phosphorylation consensus sequence was identified using WebLogo. b The Venn diagram of selected CCNY interactors (Screening A, Scr A) and CDK16/CCNY direct substrates (Screening B, Scr B) illustrates that PRC1 is the only substrate identified by both approaches. c Schematic representation of the human PRC1 locus showing the NLS domain where Thr481 is located. d In vitro kinase assay using WT-GST-CDK16, GST-CCNY and either WT or T481A-mutant of PRC1

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