Fig. 6: The proliferative action of CDK16 is mediated by PRC1 phosphorylation.

a–c Here, 293T AS-CDK16 cells were seeded and transfected the following day with siRNA targeting PRC1 or with scrambled control and treated with 0 or 5 µM 3MBPP1. a Western blot analysis was performed to confirm PRC1 downregulation. b Cell viability was monitored by an MTT assay, and the columns represent the means ± SEMs of 6 independent experiments performed in triplicate. *P < 0.05, **P < 0.01 vs control; Mann–Whitney test. c Cell proliferation was assessed by a BrdU incorporation assay 72 h after transfection. The columns represent the means ± SEMs of 4 independent experiments performed in triplicate. *P < 0.05 vs control, Mann–Whitney test. d Here, 293T AS-CDK16 cells were seeded and transfected the following day with siRNA targeting PRC1 or with scrambled control siRNA. After 3 days, the cells were detached, seeded in 6-well plates and treated with 0 or 5 µM 3MBPP1. The colony formation ability was assessed 2 weeks later. The columns represent the means ± SEMs of 4 independent experiments performed in duplicate. *P < 0.05 vs control, Mann–Whitney test