Fig. 8: Effects of exogenous regulation of the Wnt and FGF pathways on regenerative proliferation at 24 hpa and 48 hpa after neomycin injury. | Experimental & Molecular Medicine

Fig. 8: Effects of exogenous regulation of the Wnt and FGF pathways on regenerative proliferation at 24 hpa and 48 hpa after neomycin injury.

From: Wnt/β-catenin interacts with the FGF pathway to promote proliferation and regenerative cell proliferation in the zebrafish lateral line neuromast

Fig. 8

a1–G3: Regenerating cells labeled with BrdU (red) and SCs labeled with Sox2 (white). a1–a3: 24 hpa after neomycin damage; A1–A3: 48 hpa after neomycin damage; b1–b3: 24 hpa after addition of BIO, an activator of the Wnt pathway; B1–B3: 48 hpa after addition of BIO; c1–c3: 24 hpa after addition of IWR-1, an inhibitor of the Wnt pathway; C1–C3: 48 hpa after addition of IWR-1; d1–d3: 24 hpa after addition of SU5402, an inhibitor of the FGF pathway; D1–D3: 48 hpa after addition of SU5402; e1–e3: 24 hpa after addition of bFGF, an activator of the FGF pathway; E1–E3: 48 hpa after addition of bFGF; f1–f3: 24 hpa after addition of BIO and SU5402; F1–F3: 48 hpa after addition of BIO and SU5402; g1–g3: 24 hpa after addition of IWR-1 and bFGF; G1–G3: 48 hpa after addition of IWR-1 and bFGF. h–j: Quantification of the supporting cells (Sox2+) (h), proliferative cells (BrdU+) (i) and regenerating supporting cells (BrdU+ Sox2+) (j) after blocking or enhancing the Wnt and FGF pathways. n = 7 fish per group. ** Indicates p < 0.001, # indicates p < 0.05, and error bars indicate the standard error of the mean. Scale bar in G3 = 20 μm for a1–G3

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