Fig. 3: Increase in macrophage infiltration into wound sites in mS6KO mice. | Experimental & Molecular Medicine

Fig. 3: Increase in macrophage infiltration into wound sites in mS6KO mice.

From: Myeloid cell-specific sirtuin 6 deficiency delays wound healing in mice by modulating inflammation and macrophage phenotypes

Fig. 3

a Immunohistochemistry was used to identify infiltrating macrophages (F4/80+ cells) at 0, 3, and 7 days after injury. Bars = 50 μm. b F4/80-positive macrophages were counted in wound sections. c The mRNA level of F4/80 (Adgre1) in the wound site was determined by real-time RT-PCR (n = 8). d, e Single-cell suspensions were prepared by enzymatically digesting and gently dissociating skin wounds. After excluding dead cells, the cells were analyzed by flow cytometry to identify all macrophages (F4/80hiLy6gloCD11bhi) and M2 macrophages (F4/80hiLy6cloMHCIIhi) (n = 5–6). Values represent the mean ± SEM. *p < 0.05 and **p < 0.01 vs. WT

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