Fig. 6: Delayed migration of skin keratinocytes and fibroblasts in wound scratch assays. | Experimental & Molecular Medicine

Fig. 6: Delayed migration of skin keratinocytes and fibroblasts in wound scratch assays.

From: Myeloid cell-specific sirtuin 6 deficiency delays wound healing in mice by modulating inflammation and macrophage phenotypes

Fig. 6

Bone marrow macrophages (BMMs, 2 × 106) isolated from WT or KO mice were treated with TNF-α (10 ng/ml), IL-1β (10 ng/ml), and IL-6 (10 ng/ml) for 3 h, and then conditioned medium (CM) was harvested. Scratch wounds were made in monolayers of HaCaT (a) and MDFB cells (b). For rescue experiments, KO BMMs were transduced with AdSirt6. Representative images show the progression of wound closure at the indicated times. The scratch area is indicated by a dotted line. The rate of wound closure was calculated by measuring the scratch area under the same magnification at different times. Values represent the mean ± SEM (n = 8) **p < 0.01 vs. WT; #p < 0.05; and ##p < 0.01 vs. KO

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