Fig. 7: Regulation of M2 polarization by Sirt6. | Experimental & Molecular Medicine

Fig. 7: Regulation of M2 polarization by Sirt6.

From: Myeloid cell-specific sirtuin 6 deficiency delays wound healing in mice by modulating inflammation and macrophage phenotypes

Fig. 7

a BMMs were treated with 10 ng/ml IL-4 and 10 ng/ml MCSF for 6 h, and the expression patterns of M2 marker genes were compared by RT-PCR. b BMMs were treated with 10 ng/ml IL-4 for the indicated periods, and then whole-cell extracts were generated and analyzed by Western blotting. c, d BMMs from mS6KO mice were transduced with an adenovirus expressing β-galactosidase (AdLacZ), Sirt6 (AdSirt6), or CA-Akt (AdAkt) at a multiplicity of infection (MOI) of 100 and treated with IL-4 for the indicated times. Protein and mRNA levels were analyzed. e, f BMMs from WT mice were treated with 10 ng/ml IL-4 and 10 ng/ml MCSF for 6 h with or without Akt inhibitors (8 μM MK2206 and 10 μM Akti), and then the protein levels in the whole cell extract and mRNA levels of M2 marker genes were determined. Values represent the mean ± SEM (n = 3). *p < 0.05 and **p < 0.01 vs. AdLacZ or Veh; #p < 0.05 vs. IL-4. Veh Vehicle, MK MK2206, i Akti

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