Fig. 1: Deletion of p110β is restricted to SF-1 neurons of the VMH.

Expression of p110β detected by RNA in situ hybridization in the anterior VMH of a control and b p110β KO^sf1 mice, and the posterior VMH of c control and d p110β KO^sf1 mice. e mRNA expression of p110β, p110α, p85α, and PI3K-C2α in the VMH of control and p110β KO^sf1 mice. f The VMH was collected after i.p. administration of leptin (5 mg/kg body weight) and the levels of pAKT and pFoxO1 were measured in control and p110β KO^sf1 mice. The number of mice in each group is indicated in the figure. *P < 0.05 by Student’s t-test. Data are shown as mean ± SEM. Scale bar = 200 μm. Abbreviations: 3V, third ventricle; ARC, arcuate nucleus; Ctr, control; DMH, dorsomedial nucleus of the hypothalamus