Fig. 7: sip32 treatment restores vasoconstriction activity in de-endothelialized vessels from HCD-fed ApoE−/− mice. | Experimental & Molecular Medicine

Fig. 7: sip32 treatment restores vasoconstriction activity in de-endothelialized vessels from HCD-fed ApoE−/− mice.

From: Arginase II activity regulates cytosolic Ca2+ level in a p32-dependent manner that contributes to Ca2+-dependent vasoconstriction in native low-density lipoprotein-stimulated vascular smooth muscle cells

Fig. 7

De-endothelialized aortas from WT mice fed an ND and ApoE−/− mice fed an HCD for 8 weeks were incubated with sip32 and scm siRNA. Incubation of sip32 resulted in a decrease in mitochondrial protein levels (a, representative blot from three independent experiments). In a vascular tension assay using de-endothelialized aortas with vasoconstrictive agonists, PE (b) and NE (c), the decreased constriction in aortas from ApoE−/− mice fed an HCD was restored with incubation of sip32 (*, P < 0.01, **, P < 0.01). n = 4 mice. All groups showed no responses to Ach (d)

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