Fig. 4: Nox4 regulates the flagellin-induced migration of smooth muscle cells.
From: The flagellin-TLR5-Nox4 axis promotes the migration of smooth muscle cells in atherosclerosis
a Transwell migration assay. WT and Nox4 KO SMCs were seeded into the upper chamber, and stimulating medium containing flagellin (100 ng/mL) was added to the lower chamber. After 16 h, the migrated cells were stained with hematoxylin/eosin and counted. Representative images are shown for each group. b Quantification of the transwell migration assay results (N = 5, mean ± SD, *p < 0.05, ***p < 0.001). c Wound healing assay. After WT and Nox4 KO SMCs (initial cell number: 1 × 105 cells) reached 90% confluence, the cells were serum-starved for 4 h and then pretreated with mitomycin C (5 μg/mL) for 1 h. The SMCs were subjected to injury by scratching and then incubated with or without flagellin (100 ng/mL) for 24 h. d The migrated cells in the wound healing assay were quantified (N = 5, mean ± SD, ***p < 0.001). e The path length of the migrating cells after 4 h is presented as the mean ± SD from three independent experiments (n = 9 cells). **p < 0.01 (Student’s t- test)
