Fig. 1: RACK1 is upregulated during RANKL-induced osteoclastogenesis.
From: RACK1 interaction with c-Src is essential for osteoclast function
Bone marrow-derived macrophages (BMMs) were cultured with 30 ng/mL M-CSF and 100 ng/mL RANKL for the indicated period of time. a RACK1, c-Src, NFATc1, and V-ATPase d2 mRNA levels were analyzed using real-time PCR. Data are presented as the mean ± SD of three independent experiments. *P < 0.01, **P < 0.05. b RACK1, c-Src, NFATc1, and V-ATPase d2 protein levels in whole cell lysates were analyzed by western blotting with antibodies specific for the indicated proteins. The ratio of RACK1 to actin was quantified from three independent experiments. *P < 0.01, **P < 0.05
