Fig. 4: The reduced level of Opa1 in TBX18-iPMs correlates with smaller mitochondria.
From: Induced cardiac pacemaker cells survive metabolic stress owing to their low metabolic demand
a Proteomic quantification of mitochondrial fusion factors, Mfn1, Mfn2, Opa1, Oma1, and Yme1l1 in the TBX18-iPM proteome (n = 3). b Validation of Opa1 protein expression levels in D3 GFP-NRVMs and TBX18-iPMs (n = 6). c Relative mRNA expression levels of fusion-related factors, including Mfn1, Mfn2, Opa1, Oma1, and Yme1l, normalized to Gapdh in GFP-NRVMs and TBX18-iPMs (n = 6). d mRNA level of Opa1 after siOpa1 transfection in TBX18-iPMs (n = 4). e Reduced protein level of Opa1 with siOpa1 transfection in TBX18-iPMs. Representative immunoblot (left) and quantitative graph (right) (n = 4). f Mitochondrial size distribution of GFP-NRVMs and TBX18-iPMs with siScramble or siOpa1 transfection. Representative pictures of Mitotracker staining (left) and % mitochondrial size distribution divided as >10 μm2, 1–10 μm2, and 0.1–1 μm2 (right) (n = 4 for GFP-NRVMs transfected with siScramble or siOpa1 and n = 6 for TBX18-iPMs transfected with siScramble or siOpa1). g Mitochondrial density in given cytoplasmic area of GFP-NRVMs and TBX18-iPMs with siScramble or siOpa1 transfection (n = 4 for GFP-NRVMs transfected with siScramble or siOpa1 and n = 6 for TBX18-iPMs transfected with siScramble or siOpa1). *p < 0.05
