Fig. 5: Mitochondrial dynamics regulate the synchronous automaticity of TBX18-iPMs.
From: Induced cardiac pacemaker cells survive metabolic stress owing to their low metabolic demand
a BPMs of synchronous beats from siScramble- or siOpa1-transfected TBX18-iPMs on D2, D4, and D6 after transfection (n = 5). Representative distribution of synchronous beats from siScramble- or siOpa1-transfected TBX18-iPMs on D2, D4, and D6 after transfection (right three panels). b Conduction velocity of spontaneous synchronous beatings from GFP- and TBX18-NRVMs with or without Opa1 knockdown on D2, D4, and D6 after transfection (n = 5). c Relative protein levels of selected gap junctions, including phosphorylated Cx43 (p-Cx43), Cx43, and Cx45, were analyzed in D4 GFP-NRVMs and TBX18-iPMs (n = 4). Representative immunoblots are shown on the left and a quantitative graph is presented on the right. BPM: Beats per minute. Scale bar: 5 μm *p < 0.05
