Fig. 4: Effect of 6SG on in vivo models of developmental angiogenesis in the mouse retina.

a Diagram depicting the experimental schedule for the generation of in vivo models of developmental angiogenesis in the mouse retina and 6SG treatments. b Representative tiled images of retinal vasculature at two doses of 6SG (25 or 50 mg/kg). The radial lengths are drawn with dotted black lines. “S (sprout)” in red squares is magnified in d. “V (vein)” in red squares is magnified in f. Scale bar, 500 μm. c Quantitative analysis of radial length b. The graph shows the means ± SD (n = 5). ^**P < 0.01 compared with the control. d Representative magnified images of “S” in b (sprout formation) following treatment with 6SG (25 or 50 mg/kg). The sprouts are marked with red dots. Scale bar, 50 μm. e Quantitative analysis of sprout number d. The graph shows the mean ± SD (n = 5). ^**P < 0.01 compared with the control. f Representative magnified images of “V” in b (retinal vasculature) following treatment with 6SG (25 or 50 mg/kg). Branch points are marked with red dots. Scale bar, 50 μm. g, h Quantitative analysis of the number of branch points and the vascular area f. The graph shows the mean ± SD (n = 5). ^**P < 0.01 compared with the control