Fig. 2: MPP+ induces Drp1-dependent aberrant mitochondrial fission in PC12 cells. | Experimental & Molecular Medicine

Fig. 2: MPP+ induces Drp1-dependent aberrant mitochondrial fission in PC12 cells.

From: ROCK1 induces dopaminergic nerve cell apoptosis via the activation of Drp1-mediated aberrant mitochondrial fission in Parkinson’s disease

Fig. 2

a Cells were transfected with a DsRed-Mito plasmid, and the mitochondria were viewed by confocal microscopy. Scale bars: 5 μm. b Mitochondria length was quantified using the Imaris software. c PC12 cells were treated with various doses of MPP+ (0, 0.5, 1, 2, 3, and 4 mM) for 24 h. The expression of Drp1 in the mitochondrial lysates (Mito) and in the cytosolic fractions (Cyto) was determined by western blot analysis. d The stable expression of a nontargeting shRNA (shCon) or Drp1 shRNA (shDrp1) in PC12 cells was confirmed by western blot analysis. e Cells treated with MPP+ (1 mM) alone or in combination with Drp1 knockdown were transfected with a DsRed-Mito plasmid, and the mitochondria were viewed by confocal microscopy. Scale bars: 5 μm. f Mitochondrial length was quantified using the Imaris software. g The ATP Determination Kit was used to determine the concentration of ATP. h The expression of C-Cas 3 and C-PARP in whole-cell lysates was determined by western blot analysis. i, j The apoptosis rate was measured by flow cytometry using annexin V-FITC/PI staining. The data are expressed as the means ± S.D. (n = 3). *P < 0.05, ***P < 0.001

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