Fig. 2: The self-renewal, invasion, and proliferation abilities of HCC stem cells are suppressed by HOXA11-AS silencing. | Experimental & Molecular Medicine

Fig. 2: The self-renewal, invasion, and proliferation abilities of HCC stem cells are suppressed by HOXA11-AS silencing.

From: Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells

Fig. 2

a The interference efficiency of sh-HOXA11-AS in HCC stem cells as determined by RT-qPCR; b the expression of CD133, CD44, Nanog, Sox2, and Oct4 in HCC stem cells treated with sh-HOXA11-AS as measured by RT-qPCR; c gray value analysis of CD133, CD44, Nanog, Sox2, Oct4, and GAPDH bands; d the protein levels of CD133, CD44, Nanog, Sox2, and Oct4 in HCC stem cells following sh-HOXA11-AS transfection; e the tumorsphere formation ability of HCC stem cells treated with sh-HOXA11-AS as evaluated by a tumorsphere formation assay (scale bar = 100 μm); f the colony formation ability of HCC stem cells treated with sh-HOXA11-AS as evaluated by a soft agar colony formation assay; g the invasion ability of HCC stem cells treated with sh-HOXA11-AS as assessed by a Transwell assay (scale bar = 50 μm); h the proliferation ability of HCC stem cells treated with sh-HOXA11-AS as assessed by EdU staining (scale bar = 50 μm); *p < 0.05 vs. the sh-NC group. All data are measurement data and are expressed as the means ± standard errors. Comparisons of the data between two groups were performed with an unpaired t test. The experiments were repeated three times

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