Fig. 7: HOXA11 overexpression or Wnt signaling pathway inhibition reverses the promotive effect of HOXA11-AS on HCC stem cell characteristics. | Experimental & Molecular Medicine

Fig. 7: HOXA11 overexpression or Wnt signaling pathway inhibition reverses the promotive effect of HOXA11-AS on HCC stem cell characteristics.

From: Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells

Fig. 7

a The mRNA expression of CD133, CD44, Nanog, Sox2, and Oct4 in HCC stem cells as measured by RT-qPCR; b gray value analysis and protein levels of CD133, CD44, Nanog, Sox2, Oct4, and GAPDH in HCC stem cells; c gray value analysis and protein levels of HOXA11 and DNMT1 in HCC stem cells; d the tumorsphere formation ability of HCC stem cells following different treatments as evaluated by a tumorsphere formation assay (scale bar = 100 μm); e the colony formation ability of HCC stem cells as evaluated by a soft agar colony formation assay; f the invasion ability of HCC stem cells following different treatments as assessed by a Transwell assay (scale bar = 50 μm); g the proliferation ability of HCC stem cells as assessed by EdU staining (scale bar = 50 μm); *p < 0.05 vs. the oe-NC group; #p < 0.05 vs. the oe-HOXA11-AS + oe-NC group; &p < 0.05 vs. the oe-HOXA11-AS + PBS group. All data are measurement data and are expressed as the means ± standard errors; comparisons among multiple groups were performed with one-way analysis of variance. The experiments were repeated three times

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