Fig. 8: HOXA11 overexpression or Wnt signaling pathway inhibition reverses the promotive effect of HOXA11-AS on the tumorigenicity of HCC stem cells in vivo.

a The growth nude mice subcutaneously injected with HCC stem cells with different treatments; b the weights of tumors obtained from nude mice injected with HCC stem cells with different treatments; c the volumes of tumors obtained from nude mice injected with HCC stem cells with different treatments; d the mRNA expression of HOXA11, NKD1, β-catenin, c-myc, and cyclinD1 in tumors from nude mice injected with HCC stem cells with different treatments as detected by RT-qPCR; e, f the protein expression of HOXA11, NKD1, β-catenin, c-myc, and cyclinD1 in tumors from nude mice injected with HCC stem cells with different treatments as examined by western blot analysis. *p < 0.05 vs. the oe-NC group; #p < 0.05 vs. the oe-HOXA11-AS + oe-NC group; &p < 0.05 vs. the oe-HOXA11-AS + PBS group. All data are measurement data and are expressed as the means ± standard deviations. Comparisons among multiple groups were performed with one-way analysis of variance or repeated measures analysis of variance (n = 6)