Fig. 3: HIF-1α plays an important role in apoptosis induced by mitophagy via NDUFA4L2. | Experimental & Molecular Medicine

Fig. 3: HIF-1α plays an important role in apoptosis induced by mitophagy via NDUFA4L2.

From: Mitochondrial NDUFA4L2 attenuates the apoptosis of nucleus pulposus cells induced by oxidative stress via the inhibition of mitophagy

Fig. 3

Primary nucleus pulposus cells were pretreated with Dig before being exposed to TBHP. a Western blotting for the protein levels of HIF-1α, NDUFA4L2, Bcl-2/Bax, Parkin and LC-3II. b–f Quantitative analysis of the protein contents of HIF-1α, NDUFA4L2, Bcl-2/Bax, Parkin and LC-3II. g Flow cytometry was used to determine the mitochondrial membrane potential (mtΔΨ) by JC-1 staining. h Quantitative analysis of the mitochondrial membrane potential (mtΔΨ). (i) Flow cytometry was used to detect apoptosis in nucleus pulposus cells. j Quantitative analysis of flow cytometry for the detection of apoptosis. The data are presented as the mean ± S.D. ***p < 0.001, **p < 0.01, *p < 0.05 (n = 5).

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