Fig. 2: Development of epidermal hyperplasia and psoriasis-like skin inflammation in inducible transgenic mice expressing the human Peli1 gene. | Experimental & Molecular Medicine

Fig. 2: Development of epidermal hyperplasia and psoriasis-like skin inflammation in inducible transgenic mice expressing the human Peli1 gene.

From: Pellino1 promotes chronic inflammatory skin disease via keratinocyte hyperproliferation and induction of the T helper 17 response

Fig. 2

a Generation of inducible human Peli1 transgenic mice. The cDNA sequence for human Peli1 was placed under the Tet-responsive promoter and introduced into fertilized mouse oocytes. To generate rtTA-Peli1 mice, the founders were crossed with R26-M2rtTA mice (B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J). For inducibility, Myc epitope-tagged human Peli1 gene sequences under the control of the TetO promoter and human early cytomegalovirus enhancer were included. b Immunoblotting analysis of tissues derived from rtTA and rtTA-Peli1 mice using antiPeli1 and antiMyc antibodies revealing Peli1 protein expression. Doxy doxycycline treatment. c Adult rtTA control mice and rtTA-Peli1 inducible transgenic mice were treated with or without doxycycline (2 mg/mL) for 12 weeks. Offspring of three rtTA-Peli1 founder lines exhibited macroscopic pathologies after doxycycline administration. More than 95% of rtTA-Peli1 mice showed several psoriasis-like features, including extensive erythema, hair loss, severe pruritus, and loosely adherent silver-white scaling. d Clinical psoriasis scores of rtTA and rtTA-Peli1 mice treated with or without doxycycline are shown. Scores derived from 3 to 5 mice per group. Data are presented as the means ± SEMs. ***P < 0.001, two-way ANOVA. e H&E staining and immunostaining of representative skin sections.

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