Fig. 5: Deficiency of PRDX2 augments the activity of MMPs and oxidative stress in Ang II-induced AAA.
From: Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
Analysis of abdominal aortas from Prdx2+/+ and Prdx2−/− mice infused with saline or Ang II for 4 weeks. a Representative in situ zymography images and b quantification using cross-sectioned abdominal aortas (saline groups, n = 3; Ang II groups, n = 5–6). Scale bar, 50 µm. Data are presented as the mean ± SEM (two‐tailed Student’s t test). c Immunoblotting analysis of MMP2, MMP9, fibronectin, collagen IV, and PRDX2 in aortas from Prdx2+/+ and Prdx2−/− mice infused with saline or Ang II for 4 weeks. β-actin was used as a loading control. d Quantification of MMP2 and e fibronectin in Fig 5c. Data are presented as the mean ± SEM (two‐tailed Student’s t test). f Representative confocal images of DHE staining in abdominal aortic cross-sections. Scale bar, 50 µm. g Immunoblotting analysis of 4-HNE in aortas from Prdx2+/+ and Prdx2−/− mice infused with saline or Ang II for 4 weeks. β-actin was used as a loading control. h Representative immunostaining images of 4-HNE (left) and 8-OHG (right) in abdominal aortic cross-sections. Low magnification images (scale bar, 200 µm) and higher magnification of the boxed area (scale bar, 100 µm). Nuclei were stained with DAPI.
