Fig. 3: SET8 overexpression reversed high glucose-mediated endothelial inflammation.

a Results from the western blot analysis of SET8 and H4K20me1 in HUVECs, HUVEC nuclei, and HUVEC cytoplasm cultured under normal or high-glucose conditions. b qPCR analysis of SET8 in HUVECs cultured under normal or high-glucose conditions. c Results from the western blot analysis of SET8, PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs overexpressing SET8 under high-glucose conditions. d qPCR analysis of SET8, PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs overexpressing SET8 under high-glucose conditions. e Monocyte/endothelial adhesion under normal or high-glucose conditions and with SET8-overexpressing HUVECs under high-glucose conditions was measured. f Results from the western blot analysis of SET8, PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs expressing shSET8. g Results from the qPCR analysis of SET8, PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs expressing shSET8. h Monocyte/endothelial adhesion under normal conditions and in the presence of HUVECs expressing shSET8 was measured. i The results from the western blot analysis of SET8, PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs in which SET8 was silenced and PTEN was downregulated. j Results from the qPCR analysis of SET8, PTEN, e-selectin, and ICAM-1 expressions in HUVECs in which SET8 was silenced and PTEN was downregulated. k Monocyte/endothelial adhesion in the presence of HUVECs expressing shSET8 or shSET8+siPTEN was measured. *P < 0.05, compared with the control group; ^#P < 0.05, compared with the high-glucose treatment or shSET8 treatment group, n = 5/group.