Fig. 5: FOXO1 downregulation reversed high glucose-mediated endothelial inflammation.

a Results from the western blot analysis of PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs expressing siFOXO1 under high-glucose conditions. b Results from the qPCR analysis of PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs expressing siFOXO1 under high-glucose conditions. c The effects of siFOXO1 on high glucose-induced monocyte/endothelial adhesion were measured. d Results from the western blot analysis of PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs with siPTEN under FOXO1 overexpression conditions. e Results from the qPCR analysis of PTEN, p-p65, e-selectin, and ICAM-1 expressions in HUVECs with siPTEN and overexpressing FOXO1. f The effects of siPTEN on FOXO1 overexpression-induced monocyte/endothelial adhesion were measured. *P < 0.05, compared with the control group; #P < 0.05, compared with the high-glucose treatment group, n = 5/group.