Fig. 6: SET8 interacted with FOXO1 to modulate PTEN expression in HUVECs.
a H4K20me1 and FOXO1 were enriched in the PTEN promoter region. The re-ChIP assay results indicated that H4K20me1 and FOXO1 occupied the same region of the PTEN promoter. b The putative FOXO1-binding site of PTEN. The motif logo and position weight matrix are shown in the upper and lower panels, respectively. c PTEN promoter activity was detected by luciferase reporter assays. *P < 0.05, compared with the control group. d Results from the western blot analysis of SET8 and PTEN expressions in HUVECs compared with HUVECs serving as the control or those overexpressing SET8 or mutant SET8R259G. e Results from the qPCR analysis of SET8 and PTEN expressions in HUVECs compared with HUVECs serving as the control or those overexpressing SET8 or mutant SET8R259G. f Results from the western blot analysis of SET8 and PTEN expressions in HUVECs compared with HUVECs serving as the control or those transfected with shSET8. g Results from the qPCR analysis of SET8 and PTEN expressions in HUVECs compared with HUVECs serving as the control or those transfected with shSET8. h Results from the western blot analysis of SET8 and PTEN expressions in HUVECs compared with HUVECs serving as the control or those overexpressing FOXO1. i Results from the qPCR analysis of SET8 and PTEN expressions in HUVECs compared with HUVECs serving as the control or those overexpressing FOXO1. *P < 0.05, compared with the control group, n = 5/group.
