Fig. 7: The SET8 decrease and FOXO1 increase were confirmed in patients with diabetes and rats.

a The mRNA expression of SET8 was examined by qPCR in the peripheral blood mononuclear cells of patients with diabetes and healthy volunteers (con: n = 30, DM: n = 50). b The mRNA expression of FOXO1 was examined by qPCR in peripheral blood mononuclear cells from patients with diabetes and healthy volunteers (con: n = 30, DM: n = 50). c Results from the western blot analysis of SET8 and FOXO1 expressions in peripheral blood mononuclear cells in patients with diabetes and healthy volunteers (con: n = 30, DM: n = 50). d The mRNA expression of SET8 was examined by qPCR in rat aortic tissues from the control group and the diabetic group (n = 5/group). e The mRNA expression of FOXO1 was examined by qPCR in rat aortic tissues from the control group and the diabetic group (n = 5/group). f Results from the western blot analysis of SET8 and PTEN expressions in rat aortic tissues from the control group and the diabetic group (n = 5/group). g Immunostaining of SET8 and FOXO1 in rat aortic tissues from the control group and the diabetic group (n = 5/group). Scale bar, 20 μm. h Schematic representation of the working model. PBM peripheral blood mononuclear cells. *P < 0.05, compared with the control group.