Fig. 2: METTL3 increases the expression of miR-221-3p by enhancing pri-miR-221-3p m6A mRNA methylation in MCF-7/ADR cells. | Experimental & Molecular Medicine

Fig. 2: METTL3 increases the expression of miR-221-3p by enhancing pri-miR-221-3p m6A mRNA methylation in MCF-7/ADR cells.

From: METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner

Fig. 2

a, b Immunoblots and quantification of METTL3 among MCF-10A human breast epithelial cells, MCF-7/ADR cells, MCF-7/S cells, METTL3 overexpression and knockdown MCF-7/ADR cells normalized to GAPDH. c The expression of miR-221-3p was determined by RT-qPCR in METTL3 overexpression and knockdown MCF-7/ADR cells normalized to U6 expression. d, e Me-RIP assay was performed using anti-DGCR8 and anti-m6A in METTL3 overexpression and knockdown MCF-7/ADR cells, and the immunoprecipitated RNA was subjected to RT-qPCR of pri-miR-221-3p. Statistical comparisons were performed using unpaired t-test when only two groups were compared or by Tukey’s test-corrected ANOVA when more than two groups were compared. *p < 0.05 compared to MCF-10A, empty vector (oe-NC), or scramble shRNA (sh-NC) and #p < 0.05 compared to MCF-7/S cells.

Back to article page