Fig. 5: miR-221-3p promotes ADR resistance by modulating HIPK2 interaction with Che-1 in MCF-7 cells. | Experimental & Molecular Medicine

Fig. 5: miR-221-3p promotes ADR resistance by modulating HIPK2 interaction with Che-1 in MCF-7 cells.

From: METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner

Fig. 5

a The starBase database analysis of the association of HIPK2 with Che-1. b The LinkedOmics database (http://www.linkedomics.org) analysis of the association of HIPK2 with Che-1. c HIPK2 coexpressed with Che-1 by MEM database analysis (https://biit.cs.ut.ee/mem/index.cgi). d Immunoblots and quantification of Che-1 among MCF-10A human breast epithelial cells, MCF-7/ADR cells, and MCF-7/S cells normalized to GAPDH. e Immunoblots and quantification of HIPK2 and Che-1 in MCF-7/ADR cells in response to overexpression of HIPK2, Che-1, or miR-221-3p, normalized to GAPDH. f The IC50 of MCF-7/ADR cells in response to overexpression of HIPK2, Che-1, or miR-221-3p, examined by MTT assays. g The apoptosis of MCF-7/ADR cells in response to overexpression of HIPK2, Che-1, or miR-221-3p, examined by Annexin V-FITC/PI-labeled flow cytometry. h Immunoblots and quantification of BCRP, MDR1, Bcl-2 and Bax proteins in MCF-7/ADR cells in response to overexpression of HIPK2, Che-1, or miR-221-3p normalized to GAPDH. *p < 0.05 compared to MCF-10A and oe-NC, #p < 0.05 compared to oe-HIPK2 + oe-NC and MCF-7/S cells, and &p < 0.05 compared to miR-221-3p mimic + empty vector (oe-NC) by Tukey’s test-corrected ANOVA or by Bonferroni-corrected repeated measures ANOVA only for f.

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