Fig. 1: Identification of Tgf-β2 as a target gene of β-catenin in the murine uterus.
From: β-catenin activates TGF-β-induced epithelial–mesenchymal transition in adenomyosis
a Venn diagram illustrating the overlap between nonredundant genes bound by β-catenin as determined by ChIP-seq and those genes regulated by β-catenin by transcriptomic analysis (>1.5-fold) in control and mutant mouse uteri. b Quantitative real-time PCR analysis of Tgf-β2 in the uterus of control and mutant mice at 4 weeks of age (n = 3 per genotype). c Representative photomicrographs and histological score (H-score) of TGF-β2 immunohistochemical staining in uteri of control and mutant mice at 4 weeks of age (n = 5 per genotype). Scale bars represent 50 μm. d Map of the β-catenin-binding site (CBS) and negative control (NC) on the Tgf-β2 promoter. e ChIP assay using the anti-β-catenin antibody on the Tgf-β2 promoter in control and mutant mouse uteri at 4 weeks of age (n = 3). The results represent the mean ± SEM. **p < 0.01 and ***p < 0.001.
