Fig. 5: Prediction of potential metabolic functions of gut microbiota and the effects of NUC on lipid metabolism gene expression and serum metabolites in HFD-fed rats.
From: Nuciferine modulates the gut microbiota and prevents obesity in high-fat diet-fed rats
Changes in the effect of NUC supplementation on the functional potential of the gut microbiome in HFD-fed rats (a) are shown. Metabolic pathways from KEGG module predictions using 16S rRNA data with PICRUSt (n = 5 per group). The effects of NUC treatment on the mRNA expression of FAS (b), SREBP-1 (c), and PPARɑ/γ (d and e) were monitored in the liver using qRT-PCR (n = 5 for each group) in comparison with the ND group. Expression was normalized against GAPDH. PLS-DA score plot of serum samples collected from different treatment groups of rats in positive ion mode (f), OPLS-DA score plot of the ND group vs HFD group (g) and the HFD group vs HFD + NUC group (h) in positive ion mode. i Summary of pathway analysis with MetaboAnalyst, including glycerophospholipid metabolism (1), glycerolipid metabolism (2), linoleic acid metabolism (3), and retinol metabolism (4) from significantly differential metabolites. The size and color of each circle are based on the pathway impact value and P value, respectively. Values are presented as the mean ± SD (n = 5 per group). #P < 0.05, ##P < 0.01, ###P < 0.001 vs ND, *P < 0.05, **P < 0.01, ***P < 0.001 vs HFD.
