Fig. 1: miRNA200b-3p is critical for Wnt5a to promote neuronal differentiation.
a RT-qPCR analyses of Wnt5a and mi200b-3p expression with neuronal differentiation over time. b Western blot analyses of p-JNK expression in NSCs treated with Wnt5a. c RT-qPCR analyses of mi200b-3p in NSCs treated with different pathway inhibitors and then stimulated with Wnt5a. d and e Immunofluorescence staining of NSCs transfected with an mi200b-3p inhibitor and then stimulated with Wnt5a. f and g RT-qPCR and western blot analyses of neurogenic marker expression in NSCs transfected with an mi200b-3p inhibitor and then stimulated with Wnt5a. SP: JNK inhibitor; PD: ERK inhibitor; SB: p38 inhibitor; Rottlerin: PKCδ inhibitor (the data are presented as the mean ± SD from one representative experiment of three independent experiments performed in triplicate. a, bP < 0.01 compared between 3 days, 5 days, and 7 days; c, dP < 0.05 compared between 5 days and 7 days; **P < 0.01 compared between groups.).
