Fig. 3: Overexpression of RhoA suppresses the neuroinductive effects of Wnt5a/mi200b-3p. | Experimental & Molecular Medicine

Fig. 3: Overexpression of RhoA suppresses the neuroinductive effects of Wnt5a/mi200b-3p.

From: Transplantation of Wnt5a-modified NSCs promotes tissue repair and locomotor functional recovery after spinal cord injury

Fig. 3

a and b Immunofluorescence staining of NSCs transfected with LV-RhoA and then treated with Wnt5a. c and d RT-qPCR and western blot analyses of neurogenic marker expression in NSCs transfected with LV-RhoA and then treated with Wnt5a. e and f Immunofluorescence staining of NSCs transfected with the RhoA gene plasmid and treated with an mi200b-3p mimic. g and h RT-qPCR and western blot analyses of neurogenic marker expression in NSCs transfected with LV-RhoA and then treated with an mi200b-3p mimic. (The data are presented as the mean ± SD from one representative experiment of three independent experiments performed in triplicate. **P < 0.01 compared between groups.).

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