Fig. 4: In vitro differentiated M1 and M2 transcriptomes show the M1-like and M2-like features of TAMs.

a PCA grouped TAMs into a separate cluster from the reference M1 or M2 cells. Cells were colored based on the origin of the sample. b The GO terms describing each principal component show the functional difference between macrophage types (Bonferroni corrected p-value < 0.01). The top 50 genes on each principal component were analyzed by DAVID 6.8⁵³. c Two-dimensional dot plots for M1 and M2 signature evaluation were constructed for three cancer types using three different gene sets: single-cell transcriptome-derived signatures (top), bulk transcriptome-derived signatures (middle), and curated signatures (bottom). The x-axis indicates expression in the M1 signature, and the y-axis indicates expression in the M2 signature. The red line represents the simple linear regression line, and the gray area represents the confidence interval. d The M1–M2 signature 2-D plot estimates the M1-like and M2-like phenotypes of normal peritoneal macrophages.