Fig. 4: Overexpression of miR-144-3p upregulates proinflammatory cytokine/chemokine expression in human MDMs. | Experimental & Molecular Medicine

Fig. 4: Overexpression of miR-144-3p upregulates proinflammatory cytokine/chemokine expression in human MDMs.

From: MiR-144-3p is associated with pathological inflammation in patients infected with Mycobacteroides abscessus

Fig. 4

a Scatter plots with 90% density ellipses showing the rank of miR-144-3p plotted against IL1B, IL6, CXCL2, and CCL2 for all subjects (HC, n = 39; Mabc, n = 31; Mmass, n = 22). Spearman’s rank correlation determined the coefficient (r) and significance. b Human primary MDMs were transfected with mimic negative control (Control) or miR-144-3p mimic (10 nM) for 24 h and then infected with or without Mabc or Mmass (MOI = 2, for each; for 3 h). Quantitative real-time PCR analysis of IL1B, IL6, TNF, and CXCL2 expression. *p < 0.05, **p < 0.01, and ***p < 0.001. Spearman correlation (a); one-way ANOVA (b). Values represent the mean (±SD) from three independent experiments performed in duplicate (b). HC healthy controls, Mabc M. abscessus subsp. abscessus, Mmass M. abscessus subsp. massiliense.

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