Fig. 2: Increased airway inflammation and AHR in older and younger mouse models of asthma.
From: Osteopontin contributes to late-onset asthma phenotypes in adult asthma patients
a Mice at 6 and 12 weeks old underwent the induction of asthma by using OVA/Alum and were assessed for the severity of asthma according to the described protocol. Mice were assessed for b AHR to methacholine at different concentrations (0, 1.56, 3.12, 6.25, 12.5, and 25 mg/mL). ***P < 0.001 at the respective weeks, between the negative control group (C6, C12) vs the asthma group (A6, A12); ###P < 0.001 between the asthma group at 6 and 12 weeks. c Total and eosinophil counts in BALF. Mouse lung tissues were used to analyze gene expression, while BALF was measured for inflammatory cytokines. d Heatmap clustering was applied to visualize the relative changes in gene expression and levels of proinflammatory cytokines according to onset age. BALF levels of e OPN and f TGF-β1 were elevated with onset age. Data are presented as the means ± SEM, n = 10 for each group. *P < 0.05, **P < 0.01, ***P < 0.001 between the groups. AHR airway hyperresponsiveness; Alum aluminum hydroxide; Areg amphiregulin; BALF bronchoalveolar lavage fluid; CH3L1 chitinase 3-like 1; Dex dexamethasone; Egfr epidermal growth factor receptor; Eos eosinophil; IFN-γ interferon γ; IL interleukin; Lymph lymphocyte; Macro macrophage; Mon montelukast; Neu neutrophil; OPN osteopontin; poly(I:C) polyinosinic:polycytidylic acid; Spp1 secreted phosphoprotein 1; Total total cell count; TGF-β1 transforming growth factor β1; TSLP thymic stromal lymphopoietin.
