Fig. 4: Silencing of NEAT1 inhibited the expression of fibrosis factors and EMT markers in BSA-induced HK-2 cells. | Experimental & Molecular Medicine

Fig. 4: Silencing of NEAT1 inhibited the expression of fibrosis factors and EMT markers in BSA-induced HK-2 cells.

From: Long noncoding RNA NEAT1 is involved in the protective effect of Klotho on renal tubular epithelial cells in diabetic kidney disease through the ERK1/2 signaling pathway

Fig. 4

a qRT-PCR was performed to screen for NEAT1 siRNA in HK-2 cells. *p < 0.05 vs si-NC, **p < 0.01 vs si-NC, ***p < 0.001 vs si-NC. HK-2 cells were transfected with si-NEAT1 and treated with 10 mg/ml BSA for 48 h. b, c lncRNA NEAT1 expression and the mRNA levels of TGF-β1, CTGF, vimentin, and α-SMA were measured by qRT-PCR. d, e The protein levels of TGF-β1, CTGF, vimentin, and α-SMA were measured by western blots. f Expression of total and phosphorylated ERK1/2 protein was detected by western blots. *p < 0.05 vs control + si-NC, **p < 0.01 vs control + si-NC, ***p < 0.001 vs control + si-NC; #p < 0.05 vs BSA + si-NC, ##p < 0.01 vs BSA + si-NC, ###p < 0.001 vs BSA + si-NC.

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