Fig. 5: Overexpression of NEAT1 further increased the expression of fibrosis factors and EMT markers in BSA-induced HK-2 cells. | Experimental & Molecular Medicine

Fig. 5: Overexpression of NEAT1 further increased the expression of fibrosis factors and EMT markers in BSA-induced HK-2 cells.

From: Long noncoding RNA NEAT1 is involved in the protective effect of Klotho on renal tubular epithelial cells in diabetic kidney disease through the ERK1/2 signaling pathway

Fig. 5

a, b lncRNA NEAT1 expression and the mRNA levels of TGF-β1, CTGF, vimentin, and α-SMA were measured by qRT-PCR. c, d The protein levels of TGF-β1, CTGF, vimentin, and α-SMA were measured by western blots. e, f Expression of total and phosphorylated ERK1/2 protein was detected by western blots. *p < 0.05 vs control + GV417, **p < 0.01 vs control + GV417, ***p < 0.001 vs control + GV417; #p < 0.05 vs BSA + GV417, ##p < 0.01 vs BSA + GV417, ###p < 0.001 vs BSA + GV417.

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