Fig. 6: Overexpression of Klotho suppressed NEAT1 expression in BSA-induced HK-2 cells. | Experimental & Molecular Medicine

Fig. 6: Overexpression of Klotho suppressed NEAT1 expression in BSA-induced HK-2 cells.

From: Long noncoding RNA NEAT1 is involved in the protective effect of Klotho on renal tubular epithelial cells in diabetic kidney disease through the ERK1/2 signaling pathway

Fig. 6

HK-2 cells were transfected with pcDNA3.0-Klotho and treated with 10 mg/ml BSA for 48 h. a Expression of Klotho mRNA was measured by qRT-PCR. b, c The protein levels of TGF-β1, CTGF, vimentin, α-SMA, and Klotho were measured by western blots. d, e The mRNA levels of TGF-β1, CTGF, vimentin, α-SMA, and lncRNA NEAT1 were measured by qRT-PCR. f Immunofluorescence and FISH were performed to investigate the expression and location of Klotho and NEAT1 after transfection with pcDNA3.0-Klotho in BSA-treated HK-2 cells. *p < 0.05 vs control + pcDNA3.0-vector, **p < 0.01 vs control + pcDNA3.0-vector, ***p < 0.001 vs pcDNA3.0-vector; #p < 0.05 vs BSA + pcDNA3.0-vector, ##p < 0.01 vs BSA + pcDNA3.0-vector, ###p < 0.001 vs BSA + pcDNA3.0-vector.

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