Fig. 8: NEAT1 is involved in the protective effect of Klotho. | Experimental & Molecular Medicine

Fig. 8: NEAT1 is involved in the protective effect of Klotho.

From: Long noncoding RNA NEAT1 is involved in the protective effect of Klotho on renal tubular epithelial cells in diabetic kidney disease through the ERK1/2 signaling pathway

Fig. 8

RNA immunoprecipitation was performed using an anti-Klotho antibody or anti-IgG antibody as a control in BSA-treated HK-2 cells for 48 h. a lncRNA NEAT1 expression was measured by qRT-PCR. #p < 0.05 vs control, ##p < 0.01 vs control, ###p < 0.001 vs control. HK-2 cells were cotransfected with si-Klotho and si-NEAT1. b, c lncRNA NEAT1 expression and the mRNA levels of TGF-β1, CTGF, vimentin, and α-SMA were measured by qRT-PCR. d, e The protein levels of TGF-β1, CTGF, vimentin, α-SMA, and Klotho were measured by western blots. f Expression of total and phosphorylated ERK1/2 protein was detected by western blots. *p < 0.05 vs si-NC, **p < 0.01 vs si-NC, ***p < 0.001 vs si-NC; #p < 0.05 vs si-Klotho + si-NC, ##p < 0.01 vs si-Klotho + si-NC, ###p < 0.001 vs si-Klotho + si-NC.

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